Evaluation of antigen panels for ELISA monitoring of mouse colonies for antibodies to Pasteurellaceae

Lab Anim. 2006 Apr;40(2):194-9. doi: 10.1258/002367706776319051.

Abstract

Pasteurellaceae infection in mice may be monitored by the detection of serum antibody using enzyme-linked immunosorbent assay (ELISA). We re-evaluated our standard antigen panel comprising Pasteurella pneumotropica and a V-factor requiring Haemophilus species (strain H21) by studying their serological relationship with Actinobacillus muris and 'Haemophilus influenzae-murium'. Serologically, A. muris and 'H. influenzae-murium' were found to be unrelated and to differ from P. pneumotropica and Haemophilus strain H21. These four antigens were used for monitoring breeding and experimental mouse colonies for a period of four years. The addition of 'H. influenzae-murium' antigen to the standard panel of antigens significantly increased the proportion of sera and serum panels showing anti-Pasteurellaceae antibody activity, but the addition of A. muris antigen did not.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Animals, Laboratory
  • Antibodies, Bacterial / blood*
  • Antigens, Bacterial / chemistry*
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay / veterinary*
  • Mice / blood
  • Mice / immunology*
  • Pasteurellaceae / immunology
  • Pasteurellaceae / isolation & purification*
  • Pasteurellaceae Infections / blood
  • Pasteurellaceae Infections / veterinary*
  • Rodent Diseases / blood
  • Rodent Diseases / microbiology*

Substances

  • Antibodies, Bacterial
  • Antigens, Bacterial