Climbing fiber deafferentation reduces insulin-like growth factor I (IGF-I) content in cerebellum

Brain Res. 1991 Nov 15;564(2):348-51. doi: 10.1016/0006-8993(91)91476-h.

Abstract

The presence of insulin-like growth factor I (IGF-I) and its mRNA in adult rat cerebellum has recently been documented. Previous immunocytochemical studies showed prominent IGF-I-like staining in fibers around Purkinje cell somas. To determine the origin of this IGF-I input to the Purkinje cell we destroyed the inferior olivary complex by either 3-acetylpiridine administration or electrolytical lesions. In both types of lesions we found a similar significant depletion of IGF-I levels in cerebellum (40-50% of controls). No changes were found in cerebellar IGF-I receptors. These results suggest that almost half of the IGF-I content in cerebellum is provided by climbing fiber afferents arising from the inferior olivary complex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cerebellum / metabolism*
  • Cerebellum / physiology
  • Insulin-Like Growth Factor I / metabolism*
  • Male
  • Neurons, Afferent / physiology*
  • Olivary Nucleus / cytology
  • Olivary Nucleus / physiology
  • Purkinje Cells / metabolism
  • Pyridines / pharmacology
  • Radioimmunoassay
  • Rats
  • Rats, Inbred Strains
  • Receptors, Cell Surface / drug effects
  • Receptors, Cell Surface / metabolism
  • Receptors, Somatomedin
  • Stereotaxic Techniques

Substances

  • Pyridines
  • Receptors, Cell Surface
  • Receptors, Somatomedin
  • 3-acetylpyridine
  • Insulin-Like Growth Factor I