Cloning and characterization of glutathione S-transferase gene in the intertidal copepod Tigriopus japonicus and its expression after exposure to endocrine-disrupting chemicals

Mar Environ Res. 2006 Jul:62 Suppl:S219-23. doi: 10.1016/j.marenvres.2006.04.050. Epub 2006 Apr 18.

Abstract

To investigate the impacts of marine pollution on aquatic organisms, we tested the intertidal copepod Tigriopus japonicus as a model species. To analyze the copepods' responses to endocrine-disrupting chemicals (EDCs), we exposed them to two different chemicals: 4,4'-octylphenol (4,4'-OP, 12.5-100 microg/L for 2 h) and polychlorinated biphenyl (PCB, 6.25-25 microg/L for two days). 4,4'-OP was toxic, although exposure time was limited to 2h. After extracting total RNA from the exposed T. japonicus, we performed reverse transcriptase-polymerase chain reaction (RT-PCR) to determine gene expression patterns following chemical exposure. To analyze the gene expression of T. japonicus, we used glutathione S-transferase with GAPDH as an internal control. Of the genes tested using EDC-exposed samples, 4,4'-OP induced upregulation of the glutathione S-transferase (GST) gene, while PCB caused downregulation of the GST gene. These results suggest that the two EDCs act in different manners in T. japonicus.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Copepoda / drug effects
  • Copepoda / enzymology
  • Copepoda / genetics*
  • DNA Primers / chemistry
  • Endocrine Disruptors / toxicity*
  • Gene Expression Regulation, Enzymologic / drug effects*
  • Glutathione Transferase / biosynthesis
  • Glutathione Transferase / drug effects
  • Glutathione Transferase / genetics*
  • Models, Animal
  • Molecular Sequence Data
  • Phenols / administration & dosage
  • Phenols / toxicity*
  • Polychlorinated Biphenyls / administration & dosage
  • Polychlorinated Biphenyls / toxicity*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Seawater
  • Water Pollutants / toxicity

Substances

  • DNA Primers
  • Endocrine Disruptors
  • Phenols
  • Water Pollutants
  • octylphenol
  • Polychlorinated Biphenyls
  • Glutathione Transferase

Associated data

  • GENBANK/AY626240