Effects of preformed immune complexes on liver enzymes & their serum clearance in mice

Indian J Med Res. 1991 Jun:94:222-7.

Abstract

Two different performed HSA-anti-HSA immune aggregates, insoluble complex at equivalence (IC-E) and soluble complex with 5 times antigen excess (IC-S)-were administered iv in experimental mice to study their interaction with liver cells. Both complexes produced no appreciable change in the levels of liver enzymes like acid phosphatase, cathepsin D and gamma-glutamyl transferase. However, marked reduction in the level of liver pseduocholinesterase (as much as 93%) was recorded in the treated animals under identical conditions of administration of both the complexes. Hepatic uptake studies revealed that within 5 min, maximal sequestration of IC occurred within the liver (10 to 18%) and the blood (70 to 82%) when computed in terms of total injected radioactive IC. After 4 h, radioactivity dropped to 3 per cent in liver and 50-40 per cent in blood. The liver seemed to be incapable of scavenging all the serum complexes at a time. Significant consumption of serum complement occurred, when freshly prepared complexes were administered to the animals, but the reduced complement level showed a tendency to reach normalcy after 2 h. The soluble and equivalence zone IC failed to exhibit identifiable discrimination facets with respect to handling by liver. The complexes IC-E and IC-S also behaved in a similar manner.

MeSH terms

  • Acid Phosphatase / metabolism
  • Animals
  • Antigen-Antibody Complex / metabolism
  • Antigen-Antibody Complex / pharmacology*
  • Butyrylcholinesterase / metabolism
  • Cathepsin D / metabolism
  • Humans
  • Injections, Intravenous
  • Liver / enzymology*
  • Liver / metabolism
  • Mice
  • Mice, Inbred BALB C
  • gamma-Glutamyltransferase / metabolism

Substances

  • Antigen-Antibody Complex
  • gamma-Glutamyltransferase
  • Butyrylcholinesterase
  • Acid Phosphatase
  • Cathepsin D