Inactivation of HIV-1 by trypsin and its use in demonstrating specific virus infection of cells

J Virol Methods. 1991 Jun;33(1-2):39-46. doi: 10.1016/0166-0934(91)90005-k.

Abstract

HIV-1 was found sensitive to inactivation by low concentrations of trypsin. The use of trypsin was valuable for assessing non-specific binding of HIV virions to CD4+ cells. This effect was also helpful for eliminating input virus in experiments studying HIV infection of cells in vitro.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antiviral Agents / pharmacology
  • CD4-Positive T-Lymphocytes / drug effects
  • CD4-Positive T-Lymphocytes / microbiology
  • Cell Line
  • Cell Membrane / microbiology
  • HIV Infections / microbiology
  • HIV-1 / drug effects*
  • Humans
  • Trypsin / pharmacology*
  • Virology / methods

Substances

  • Antiviral Agents
  • Trypsin