Immunomagnetic indirect positive sorting of precartilaginous stem cells from neonatal rat

J Huazhong Univ Sci Technolog Med Sci. 2006;26(6):723-4. doi: 10.1007/s11596-006-0626-0.

Abstract

To investigate the technique of sorting high-purity precartilaginous stem cells from rat's perichondrium, neonatal rat's perichondrium cells suspensions were incubated with monoclone antibody of anti-fibroblast growth factor receptor-3 (anti-FGFR-3), and the labeled cells were separated from the suspension in the magnetic field by immuno-beads coated with the second antibody. Purity of the sorted neural stem cells was found to be 93.0%-99.0%, with living cells amounting to 80% -85 %. The magnetic cell sorting system could effectively separate precartilaginous stem cells from perichondrium cell suspensions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Cartilage / cytology*
  • Cells, Cultured
  • Immunomagnetic Separation / methods*
  • Rats
  • Rats, Sprague-Dawley
  • Stem Cells / cytology*