Isolation of a human chromosome 14-only somatic cell hybrid: analysis using Alu and LINE-based PCR

A Mares Jr; S A Ledbetter; D H Ledbetter; R Roberts; J F Hejtmancik

doi:10.1016/0888-7543(91)90122-u

Isolation of a human chromosome 14-only somatic cell hybrid: analysis using Alu and LINE-based PCR

Genomics. 1991 Sep;11(1):215-8. doi: 10.1016/0888-7543(91)90122-u.

Authors

A Mares Jr¹, S A Ledbetter, D H Ledbetter, R Roberts, J F Hejtmancik

Affiliation

¹ Department of Medicine, Baylor College of Medicine, Houston, Texas 77030.

PMID: 1765380
DOI: 10.1016/0888-7543(91)90122-u

Abstract

Interspecific somatic cell hybrids containing single human chromosomes are valuable reagents for localization of cloned genes and DNA fragments to specific chromosomes, for the development of chromosome-specific libraries, and for generation of hybrid cell lines containing subchromosomal regions. A CHO somatic cell hybrid containing a single, intact human chromosome 14 (MHR14) was developed and confirmed by LINE PCR amplification gel pattern, by Alu-517 PCR product dot blot hybridization, and by cytogenetic analysis. MHR14 will serve as the chromosome source for the development of a radiation map of human chromosome 14.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Animals
Base Sequence
CHO Cells
Cell Separation
Chromosome Banding
Chromosomes, Human, Pair 14*
Cricetinae
DNA
Humans
Hybrid Cells*
Molecular Sequence Data
Polymerase Chain Reaction
Repetitive Sequences, Nucleic Acid

Substances

DNA