DNA typing of cytological samples for retrospective identification of an early case of Panton-Valentine leucocidin-positive, community-associated methicillin-resistant Staphylococcus aureus pneumonia

J Clin Microbiol. 2008 Jul;46(7):2457-8. doi: 10.1128/JCM.00283-08. Epub 2008 May 21.

Abstract

This paper describes a fatal case of culture-confirmed, community-associated methicillin-resistant Staphylococcus aureus (MRSA) pneumonia in an 8-month-old child in Hong Kong in 2001. Stored cytological materials prepared from the pleural fluid were retrieved for molecular analysis. The result indicates the presence of a Panton-Valentine leucocidin-positive, spa type 019 MRSA.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Toxins / genetics*
  • Community-Acquired Infections / microbiology*
  • DNA Fingerprinting
  • Exotoxins / genetics*
  • Fatal Outcome
  • Hong Kong
  • Humans
  • Infant
  • Leukocidins / genetics*
  • Methicillin Resistance*
  • Pneumonia / microbiology*
  • Staphylococcal Infections / microbiology*
  • Staphylococcus aureus / drug effects
  • Staphylococcus aureus / genetics*
  • Staphylococcus aureus / isolation & purification*

Substances

  • Bacterial Toxins
  • Exotoxins
  • Leukocidins
  • Panton-Valentine leukocidin