Frequency and distribution of Epstein-Barr virus infection and its association with P53 expression in a series of primary nodal non-Hodgkin lymphoma patients from South India

Int J Lab Hematol. 2010 Feb;32(1 Pt 2):56-64. doi: 10.1111/j.1751-553X.2008.01125.x. Epub 2009 Dec 4.

Abstract

This study investigated the prevalence of Epstein-Barr virus (EBV) infection and its association with P53 expression in a panel of 87 previously untreated nodal non-Hodgkin lymphomas (NHLs) from India. Polymerase chain reaction specific for Epstein Barr nuclear antigen 1 (EBNA1) and EBNA-3C was performed on the lymphnode tissue DNA, while P53 expression was analyzed by immunohistochemistry. EBV, predominantly type A strain, was detected in 27/87 (31%) nodal lymphoid malignancies, 11/46 diffuse large B-cell lymphomas, 6/17 follicular lymphoma, 4/6 anaplastic large cell lymphomas (ALCL), 5/11 peripheral T-cell lymphomas (PTCL) and 1/7 lymphoblastic lymphomas. EBV infection was more frequently observed in a specific subset of nodal NHL, suggesting a causative role of EBV infection in the pathogenesis of ALCL and PTCL. There was no significant association between EBV and P53 expression in our series of NHL patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Child
  • Epstein-Barr Virus Infections / metabolism*
  • Epstein-Barr Virus Nuclear Antigens / genetics
  • Female
  • Gene Expression Regulation*
  • Humans
  • Immunohistochemistry
  • India
  • Lymphoma, Non-Hodgkin* / metabolism
  • Lymphoma, Non-Hodgkin* / virology
  • Male
  • Middle Aged
  • Mutation
  • Sequence Analysis
  • Tumor Suppressor Protein p53 / genetics
  • Tumor Suppressor Protein p53 / metabolism*

Substances

  • Epstein-Barr Virus Nuclear Antigens
  • Tumor Suppressor Protein p53
  • EBV-encoded nuclear antigen 1