Studies toward the analysis of S-nitrosoproteins

Rajib Sengupta; Timothy R Billiar; Detcho A Stoyanovsky

doi:10.1039/b817981f

Studies toward the analysis of S-nitrosoproteins

Org Biomol Chem. 2009 Jan 21;7(2):232-4. doi: 10.1039/b817981f. Epub 2008 Nov 19.

Authors

Rajib Sengupta¹, Timothy R Billiar, Detcho A Stoyanovsky

Affiliation

¹ Department of Surgery, University of Pittsburgh Medical School, 3459 Fifth Ave, Pittsburgh, PA 15213, USA.

PMID: 19109666
DOI: 10.1039/b817981f

Abstract

An immuno-spin trapping method for the analysis of S-nitrosoproteins based on the reaction of protein thiyl radicals with 5,5-dimethyl-1-pyrroline N-oxide has been developed.

MeSH terms

Cyclic N-Oxides / chemistry
Nitric Oxide / chemistry
Proteins / chemistry*
S-Nitrosoglutathione / chemistry
S-Nitrosothiols / chemistry*
Spin Labels
Spin Trapping

Substances

Cyclic N-Oxides
Proteins
S-Nitrosothiols
Spin Labels
Nitric Oxide
S-Nitrosoglutathione
5,5-dimethyl-1-pyrroline-1-oxide