Qualitative and quantitative proteomic profiling of cripto(-/-) embryonic stem cells by means of accurate mass LC-MS analysis

J Proteome Res. 2009 Feb;8(2):1047-58. doi: 10.1021/pr800485c.

Abstract

Cripto is one of the key regulators of embryonic stem cells (ESCs) differentiation into cardiomyocites vs neuronal fate. Cripto(-/-) murine ESCs have been utilized to investigate the molecular mechanisms underlying early events of mammalian lineage differentiation. 2D/LC-MS/MS and a label-free LC-MS approaches were used to qualitatively and quantitatively profile the cripto(-/-) ESC proteome, providing an integral view of the alterations induced in stem cell functions by deleting the cripto gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation
  • Cells, Cultured
  • Chromatography, Liquid / methods*
  • Embryonic Stem Cells / chemistry*
  • Embryonic Stem Cells / cytology
  • Embryonic Stem Cells / physiology*
  • Epidermal Growth Factor* / genetics
  • Epidermal Growth Factor* / metabolism
  • Mass Spectrometry / methods*
  • Membrane Glycoproteins* / genetics
  • Membrane Glycoproteins* / metabolism
  • Mice
  • Mice, Knockout
  • Molecular Sequence Data
  • Neoplasm Proteins* / genetics
  • Neoplasm Proteins* / metabolism
  • Neurons / physiology
  • Protein Array Analysis / methods*
  • Proteome / analysis
  • Reproducibility of Results

Substances

  • Membrane Glycoproteins
  • Neoplasm Proteins
  • Proteome
  • Tdgf1 protein, mouse
  • Epidermal Growth Factor