Construction and characterization of a high activity mutant of human keratinocyte growth factor-2

Biotechnol Lett. 2009 Jun;31(6):797-802. doi: 10.1007/s10529-009-9948-x. Epub 2009 Feb 18.

Abstract

Keratinocyte growth factor-2 (KGF-2) plays an important role in vertebrate limb development, lung branching morphogenesis, regeneration and reconstruction of the epidermis. Previous studies have used the wild type factor. Here, we have constructed a double-site mutant of human KGF-2, named STEA. STEA possesses higher receptor binding affinity and promotes better proliferation activity on rat tracheal epithelium (RTE) cells than recombinant human KGF-2. These results suggest that the simultaneous mutation of Ser115 to Thr and Glu117 to Ala improves the biological activity of KGF-2.

MeSH terms

  • Amino Acid Substitution
  • Animals
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Epithelial Cells / drug effects
  • Fibroblast Growth Factor 10 / genetics
  • Fibroblast Growth Factor 10 / pharmacology*
  • Humans
  • Intercellular Signaling Peptides and Proteins / genetics
  • Intercellular Signaling Peptides and Proteins / pharmacology*
  • Mutation, Missense
  • Rats
  • Recombinant Proteins / genetics
  • Recombinant Proteins / pharmacology
  • Respiratory Mucosa / drug effects

Substances

  • Fibroblast Growth Factor 10
  • Intercellular Signaling Peptides and Proteins
  • Recombinant Proteins