Objective: To study the effect of Survivin antisense oligodeoxgnucleotide (ASODN) on drug resistance and its mechanism in K562 cells.
Methods: Cells were divided into four groups: ASODN group, SODN group, Lip group and blank group. Survivin ASODN was transfered into K562 cells by liposomal reagent. The expression of Survivin mRNA was detected by RT-PCR. The sensitivity of K562 cells to adriamycin (ADM) and daunorubicin (DAM) was detected by MTT assay, while the intracellular concentration of ADM and DAM was measured by flowcytometry.
Results: Compared to control group Survivin mRNA expression in ASODN group decreased obviously. IC50(s) of ADM and ASODN+ADM were 0.5457 mg/L and 0.1933 mg/L respectively, IC50 (s) of DAM and ASODN+DAM were 0.5408 mg/L and 0.2027 mg/L respectively. Expression of Survivin mRNA decreased by 25.8% after the transfection of ASODN. Fluorescence intensity of ADM and ASODN+ADM in K562 cells were 51.64 and 89.92, Fluorescence intensity of DAM and ASODN+DAM in K562 cells were 63.71 and 88.47.
Conclusion: Expression of Survivin mRNA in k562 cells was down-regulated by ASODN. Survivin ASODN is able to reverse the drug resistance via inhibition of Survivin expression and inducement of apoptosis.