Utility and evaluation of new variable-number tandem-repeat systems for genotyping mycobacterial tuberculosis isolates

Horng-Yunn Dou; Jang-Jih Lu; Chih-Wei Lin; Jia-Ru Chang; Jun-Ren Sun; Ih-Jen Su

doi:10.1016/j.mimet.2009.01.007

Utility and evaluation of new variable-number tandem-repeat systems for genotyping mycobacterial tuberculosis isolates

J Microbiol Methods. 2009 Apr;77(1):127-9. doi: 10.1016/j.mimet.2009.01.007. Epub 2009 Jan 20.

Authors

Horng-Yunn Dou¹, Jang-Jih Lu, Chih-Wei Lin, Jia-Ru Chang, Jun-Ren Sun, Ih-Jen Su

Affiliation

¹ Division of Clinical Research, National Health Research Institutes, Zhunan, Taiwan.

PMID: 19386225
DOI: 10.1016/j.mimet.2009.01.007

Abstract

We compared mycobacterial interspersed repetitive unit (MIRU)-variable number tandem repeat (VNTR) typing to traditional spoligotyping for discriminating Mycobacterium tuberculosis (MTB) strains. Our 17-loci MIRU-VNTR typing method was found to be superior to spoligotyping for non-Beijing family strains. To extend the method we also established PCR-based rapid genotyping protocols for Beijing, East-African-Indian and U lineages.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Bacterial Typing Techniques / methods*
DNA Fingerprinting / methods*
Genotype
Humans
Minisatellite Repeats*
Mycobacterium tuberculosis / classification
Mycobacterium tuberculosis / genetics
Mycobacterium tuberculosis / isolation & purification*
Polymerase Chain Reaction / methods
Tuberculosis, Pulmonary / microbiology*