Live-cell imaging of the cytoskeleton and mitochondrial-cytoskeletal interactions in budding yeast

Methods Mol Biol. 2009:586:41-68. doi: 10.1007/978-1-60761-376-3_3.

Abstract

This chapter describes labeling methods and optical approaches for live-cell imaging of the cytoskeleton and of a specific organelle-cytoskeleton interaction in budding yeast.

MeSH terms

  • Actins / metabolism
  • Carbocyanines / metabolism
  • Coloring Agents / metabolism
  • Cytoskeleton / genetics
  • Cytoskeleton / metabolism*
  • Cytoskeleton / ultrastructure
  • Fluorescent Antibody Technique
  • Fluorescent Dyes / metabolism
  • Green Fluorescent Proteins / metabolism
  • Indoles / metabolism
  • Membrane Glycoproteins / metabolism
  • Microfilament Proteins / metabolism
  • Microtubules / genetics
  • Microtubules / metabolism*
  • Microtubules / ultrastructure
  • Mitochondria / genetics
  • Mitochondria / metabolism*
  • Mitochondria / ultrastructure
  • Pyridinium Compounds / metabolism
  • Rhodamine 123 / metabolism
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae / ultrastructure
  • Staining and Labeling / methods
  • Tropomyosin / metabolism

Substances

  • Actins
  • Carbocyanines
  • Coloring Agents
  • Fluorescent Dyes
  • Indoles
  • Membrane Glycoproteins
  • Microfilament Proteins
  • Pyridinium Compounds
  • Tropomyosin
  • plastin
  • Green Fluorescent Proteins
  • Rhodamine 123
  • 2-(4-(dimethylamino)styryl)-1-methylpyridinium
  • DAPI
  • 3,3'-dihexyl-2,2'-oxacarbocyanine