Beta-catenin signaling levels in progenitors influence the laminar cell fates of projection neurons

J Neurosci. 2009 Oct 28;29(43):13710-9. doi: 10.1523/JNEUROSCI.3022-09.2009.

Abstract

The mechanisms underlying the timing of the laminar fate decisions during cortical neurogenesis remain poorly understood. Here we show that beta-catenin signaling in cortical neural precursors can regulate the laminar fate of their daughters. In ventricular zone neural precursors, beta-catenin signaling is higher when deep-layer neurons are being generated and lower when upper-layer neurons are being generated. Overactivation of beta-catenin in cortical precursors midway through corticogenesis increased the relative production of deep-layer neurons, while inhibition of signaling increased the relative production of upper-layer neurons. Furthermore, in late-gestation upper-layer precursors, overactive beta-catenin signaling was able to partially restore production of deep-layer neurons. These observations suggest that increased beta-catenin signaling can reset the timing of cortical precursors to promote the production of deep-layer neurons, while inhibition of beta-catenin signaling advances the timing to promote upper-layer production.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Axons / physiology
  • Cerebral Cortex / anatomy & histology
  • Cerebral Cortex / embryology*
  • Cerebral Cortex / physiology*
  • Electroporation
  • Mice
  • Mice, Transgenic
  • Neurogenesis / physiology*
  • Neurons / physiology*
  • Signal Transduction
  • Stem Cell Niche / embryology
  • Stem Cell Niche / physiology
  • Stem Cells / physiology*
  • Time Factors
  • beta Catenin / genetics
  • beta Catenin / metabolism*

Substances

  • beta Catenin