Multiplex PCR assay to facilitate identification of the recently described Staphylococcal cassette chromosome mec type VIII

Mol Cell Probes. 2010 Aug;24(4):229-32. doi: 10.1016/j.mcp.2010.01.001. Epub 2010 Jan 14.

Abstract

We recently described a novel staphylococcal cassette chromosome mec (SCCmec) element, designated type VIII. We report here a multiplex PCR assay, capable of identifying SCCmec type VIII. This assay will facilitate identification of this new SCCmec type and provides a useful tool for clinical and epidemiologic studies in this regard.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Biological Assay
  • Chromosomes, Bacterial / genetics*
  • Interspersed Repetitive Sequences / genetics*
  • Polymerase Chain Reaction / methods*
  • Reproducibility of Results
  • Staphylococcus / classification
  • Staphylococcus / genetics*
  • Staphylococcus / isolation & purification*