Abstract
The mitochondrial degradosome (mtEXO) is the main enzymatic complex in RNA degradation, processing, and surveillance in Saccharomyces cerevisiae mitochondria. It consists of two nuclear-encoded subunits: the ATP-dependent RNA helicase Suv3p and the 3' to 5' exoribonuclease Dss1p. The two subunits depend on each other for their activity; the complex can therefore be considered as a model system for the cooperation of RNA helicases and exoribonucleases in RNA degradation. All the three activities of the complex (helicase, ATPase, and exoribonuclease) can be studied in vitro using recombinant proteins and protocols presented in this chapter.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adenosine Triphosphatases / genetics
-
Adenosine Triphosphatases / metabolism*
-
Biological Assay / methods*
-
Endoribonucleases / chemistry
-
Endoribonucleases / metabolism*
-
Exoribonucleases / genetics
-
Exoribonucleases / metabolism*
-
Mitochondria / enzymology*
-
Multienzyme Complexes / chemistry
-
Multienzyme Complexes / metabolism*
-
Polyribonucleotide Nucleotidyltransferase / chemistry
-
Polyribonucleotide Nucleotidyltransferase / metabolism*
-
Protein Subunits / genetics
-
Protein Subunits / metabolism
-
RNA Helicases / chemistry
-
RNA Helicases / genetics
-
RNA Helicases / metabolism*
-
Recombinant Proteins / genetics
-
Recombinant Proteins / metabolism
-
Saccharomyces cerevisiae / cytology
-
Saccharomyces cerevisiae / metabolism
-
Saccharomyces cerevisiae Proteins / genetics
-
Saccharomyces cerevisiae Proteins / metabolism
Substances
-
Multienzyme Complexes
-
Protein Subunits
-
Recombinant Proteins
-
Saccharomyces cerevisiae Proteins
-
degradosome
-
Polyribonucleotide Nucleotidyltransferase
-
Endoribonucleases
-
Exoribonucleases
-
Adenosine Triphosphatases
-
RNA Helicases