Role of collapsin response mediator protein-2 in neurite outgrowth of PC12 cells

Neuroreport. 2010 Jun 23;21(9):641-5. doi: 10.1097/WNR.0b013e32833a7d53.

Abstract

Collapsin response mediator proteins (CRMPs) are involved in cell differentiation and axonal growth. In this study, we investigated neurite outgrowth and the expression of CRMP-2 in PC12 cells. Nondifferentiated PC12 cells hardly express CRMP-2, but the expression of CRMP-2 increases with neurite outgrowth. We established a stable CRMP-2-overexpressing PC12 cell line and found that PC12 cells, which constitutively overexpress CRMP-2, were unable to extend neurites after stimulation with the nerve growth factor or the neural cell adhesion molecule, a procedure that promotes neurite outgrowth in untransfected cells. In contrast, a PC12 cell line deficient in CRMP-2 extends neurites after the stimulation of nerve growth factor or neural cell adhesion molecule.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Cell Count
  • Cell Enlargement
  • Cell Line, Tumor
  • Cell Proliferation
  • Coculture Techniques
  • Humans
  • Intercellular Signaling Peptides and Proteins / deficiency
  • Intercellular Signaling Peptides and Proteins / genetics
  • Intercellular Signaling Peptides and Proteins / metabolism*
  • Mice
  • Nerve Growth Factor / metabolism
  • Nerve Tissue Proteins / deficiency
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • Neural Cell Adhesion Molecules / genetics
  • Neural Cell Adhesion Molecules / metabolism
  • Neurites / physiology*
  • PC12 Cells
  • Rats
  • Transfection

Substances

  • Intercellular Signaling Peptides and Proteins
  • Nerve Tissue Proteins
  • Neural Cell Adhesion Molecules
  • collapsin response mediator protein-2
  • Nerve Growth Factor