Exploring beyond viral load testing for EBV lymphoproliferation: role of serum IL-6 and IgE assays as adjunctive tests

Pediatr Transplant. 2010 Nov;14(7):852-8. doi: 10.1111/j.1399-3046.2010.01352.x.

Abstract

We examined serum IL-6 and IgE assays as adjuncts to VL monitoring for PTLD. Paediatric solid organ transplant recipients were followed with VL monitoring. VL, IL-6, and IgE assays were compared between PTLD cases and non-cases at <3, 3-6 and >6 months after transplantation. Median IL-6 levels in PTLD cases were 15.5 (2.0-87.1) and 23.3 (2.1-276) pg/mL compared with 3.25 (0.92-114) and 3.5 (0.75-199.25) pg/mL in non-cases at 3-6 and >6 months, respectively (p = 0.006 and p = 0.005). At >6 months, IL-6 levels correlated with VL and PTLD occurrence (Spearman's coefficients = 0.40; p = 0.001 and 0.32; p = 0.003) in univariate analyses. No benefit was derived from performance of IgE levels. The sensitivity and specificity of high VL as a test of PTLD were 76.3% and 92.5%, while the negative predictive value and PPV of VL were 94.9% and 68.4%, respectively. Combining elevated IL-6 with high VL increased the PPV and specificity to 80% and 96.2%, respectively, and improved the receiver operating characteristic curve. Serum IL-6 levels can improve the clinician's ability to identify PTLD, among patients with elevated EBV viral loads.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Area Under Curve
  • Biomarkers / metabolism
  • Child
  • Child, Preschool
  • Cohort Studies
  • Female
  • Herpesvirus 4, Human / metabolism*
  • Humans
  • Immunoglobulin E / blood*
  • Infant
  • Interleukin-6 / blood*
  • Leukocytes, Mononuclear / cytology
  • Lymphocytes / metabolism
  • Lymphocytes / virology*
  • Lymphoproliferative Disorders / blood*
  • Lymphoproliferative Disorders / virology*
  • Male
  • Prospective Studies
  • Sensitivity and Specificity
  • T-Lymphocytes, Cytotoxic / cytology
  • Viral Load

Substances

  • Biomarkers
  • Interleukin-6
  • Immunoglobulin E