Incidence and significance of FLT3-ITD and NPM1 mutations in patients with normal karyotype acute myeloid leukaemia

Ir J Med Sci. 2010 Dec;179(4):507-10. doi: 10.1007/s11845-010-0567-2. Epub 2010 Aug 31.

Abstract

Background: Acute myeloid leukaemia (AML) is a heterogeneous clonal disorder of haematopoietic progenitor cells. Approximately half of all adult AML patients have a normal karyotype (NK-AML) and an intermediate risk prognosis.

Aims: To determine the incidence and prognostic significance of NPM1 and FLT3-ITD mutations in a population of patients with NK-AML.

Methods: FLT3-ITD and NPM1 mutation status was retrospectively sought in presentation samples from 44 NK-AML patients.

Results: FLT3-ITD and NPM1 mutations were detected in 45.5 and 54.5% of patients, respectively, allowing stratification according to genotype.

Conclusions: FLT3-ITD and NPM1 mutation status can be defined in NK-AML. Prospective screening for these mutations is advocated in all NK-AML patients, as the genotype is of clinical importance when considering treatment options including stem cell transplantation.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Electrophoresis, Agar Gel
  • Female
  • Genotype
  • Humans
  • Leukemia, Myeloid, Acute / genetics*
  • Male
  • Middle Aged
  • Mutation
  • Nuclear Proteins / genetics*
  • Nucleophosmin
  • Phosphoproteins / genetics*
  • Prognosis
  • Retrospective Studies
  • Seroepidemiologic Studies
  • XYY Karyotype
  • Young Adult
  • fms-Like Tyrosine Kinase 3 / genetics*

Substances

  • NPM1 protein, human
  • Nuclear Proteins
  • Phosphoproteins
  • Nucleophosmin
  • FLT3 protein, human
  • fms-Like Tyrosine Kinase 3