1H, 13C and 15N resonance assignments of SARS-CoV main protease N-terminal domain

Biomol NMR Assign. 2011 Oct;5(2):143-5. doi: 10.1007/s12104-010-9287-9. Epub 2010 Dec 23.

Abstract

The main protease (M(pro)) of severe acute respiratory syndrome coronavirus (SARS-CoV) plays an essential role in the extensive proteolytic processing of the viral polyproteins (pp1a and pp1ab), and it is an important target for anti-SARS drug development. SARS-CoV M(pro) is composed of a catalytic N-terminal domain and an α-helical C-terminal domain linked by a long loop. Even though the N-terminal domain of SARS-CoV M(pro) adopts a similar chymotrypsin-like fold as that of piconavirus 3C protease, the extra C-terminal domain is required for SARS-CoV M(pro) to be enzymatically active. Here, we reported the NMR assignments of the SARS-CoV M(pro) N-terminal domain alone, which are essential for its solution structure determination.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbon Isotopes
  • Coronavirus 3C Proteases
  • Cysteine Endopeptidases / chemistry*
  • Nitrogen Isotopes
  • Nuclear Magnetic Resonance, Biomolecular*
  • Severe acute respiratory syndrome-related coronavirus / chemistry*
  • Viral Proteins / chemistry*

Substances

  • Carbon Isotopes
  • Nitrogen Isotopes
  • Viral Proteins
  • Cysteine Endopeptidases
  • Coronavirus 3C Proteases