Abstract
The subunit composition of the NAD(P)H dehydrogenase complex of Thermosynechococcus elongatus was analyzed by different types of mass spectrometry. All 15 known subunits (NdhA-NdhO) were identified in the purified NDH-1L complex. Moreover, two additional intact mass tags of 4902.7 and 4710.5 Da could be assigned after reannotation of the T. elongatus genome. NdhP and NdhQ are predicted to contain a single transmembrane helix each, and homologues are apparent in other cyanobacteria. Additionally, ndhP is present in some cyanophages in a cluster of PSI genes and exhibits partial similarity to NDF6, a subunit of the plant NDH-1 complex.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Bacterial Proteins / physiology
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Chromatography, High Pressure Liquid
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Cyanobacteria / chemistry
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Cyanobacteria / enzymology*
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Cyanobacteria / metabolism
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Models, Biological
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Molecular Sequence Data
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Molecular Weight
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NADH Dehydrogenase / chemistry*
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NADH Dehydrogenase / isolation & purification*
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NADH Dehydrogenase / metabolism*
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NADH Dehydrogenase / physiology
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Protein Binding
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Protein Subunits / chemistry
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Protein Subunits / isolation & purification
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Protein Subunits / metabolism
Substances
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Bacterial Proteins
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Protein Subunits
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NADH Dehydrogenase