Objective: To research the absorbed character of piperine in Erxiekang plaster, and piperine by transdermal absorption was determined.
Method: The percutaneous absorption of piperine in vitro at different times was conducted by Franz osmosis and diffusion apparatus as well as high-performance liquid chromatography.
Result: It showed that the piperine through skins of mice gradually increased within the experiment time. After 52 h, the penetration of piperine was 78.51%, and remained basically unchanged.
Conclusion: The method is reliable, and can be used for Erxiekang plaster of determination of transdermal absorption.