Abstract
Methods and procedures in molecular biology used to study fungal pathogenesis have significantly improved during the last decade. In this chapter, we provide step-by-step procedures for performing genetics and biochemical studies in the human pathogenic fungal microorganism Cryptococcus neoformans (Cn). These methods are employed for studying the pathobiology of Cn and for experimental validation of theoretical models of fungal pathogenicity.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Biolistics
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Blotting, Southern
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Chromatography, Thin Layer
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Cryptococcus neoformans / cytology*
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Cryptococcus neoformans / metabolism
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DNA, Fungal / isolation & purification
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Enzyme Assays
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Fungal Proteins / isolation & purification
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Humans
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Hydrolysis
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Lipids / chemistry
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Lipids / isolation & purification
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Mass Spectrometry
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RNA, Fungal / isolation & purification
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Systems Biology / methods*
Substances
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DNA, Fungal
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Fungal Proteins
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Lipids
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RNA, Fungal