Rhesus monkey TRIM5α has distinct HIV-1 restriction activity among different mammalian cell lines

Curr Microbiol. 2011 Dec;63(6):531-7. doi: 10.1007/s00284-011-0009-z. Epub 2011 Sep 24.

Abstract

Rhesus monkey TRIM5α (TRIM5α(rh)), a member of the tripartite motif (TRIM) family, was identified as the main restriction factor responsible for resistance of old world monkey cells to HIV-1 infection. However, the precise mechanism of HIV-1 infection inhibition by TRIM5α remains elusive and appears to be related to some cellular cofactors. Here we reported that TRIM5α(rh) can significantly reduce the infection efficiency of VSV-G pseudotyped HIV-1/MA-YFP virus in human epithelial carcinoma (HeLa) cells, moderately reduce in porcine kidney (PK-15) cells and have no effect on the pseudotyped virus infection in Madin-Darby canine kidney (MDCK) cells. Furthermore, we found that the different HIV-1 restriction activities have no relation with the intracellular localization of TRIM5α(rh). These results indicate that the cellular environment is very important for the efficient anti-HIV-1 activity of TRIM5α(rh). We speculate that some unknown factors required for HIV-1 infection inhibition activity are adequately expressed in HeLa cells, inadequately expressed in PK-15 cells and absent in MDCK cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Callithrix
  • Cloning, Molecular
  • Dogs
  • Genetic Variation
  • HIV Infections / immunology*
  • HIV Infections / virology
  • HIV-1 / genetics
  • HIV-1 / immunology*
  • HeLa Cells
  • Humans
  • Macaca mulatta
  • Microscopy, Confocal
  • Microscopy, Fluorescence
  • Molecular Sequence Data
  • Proteins / genetics
  • Proteins / immunology*
  • Species Specificity
  • Transduction, Genetic
  • Ubiquitin-Protein Ligases

Substances

  • Proteins
  • TRIM5(alpha) protein, rhesus monkey
  • Ubiquitin-Protein Ligases