SPT6, an essential gene that affects transcription in Saccharomyces cerevisiae, encodes a nuclear protein with an extremely acidic amino terminus

Mol Cell Biol. 1990 Sep;10(9):4935-41. doi: 10.1128/mcb.10.9.4935-4941.1990.

Abstract

SPT6 is an essential gene of Saccharomyces cerevisiae that appears to play a role in transcription. Mutations in the SPT6 (SSN20, CRE2) gene suppress delta insertion mutations in the 5' regions of HIS4 and LYS2 and mutations in cis- and/or trans-acting elements that are required for expression of SUC2 and ADH2. We report here that SPT6 encodes a 170-kilodalton highly charged protein with an extremely acidic amino terminus. By use of an epitope-tagged SPT6 protein, we have determined by indirect immunofluorescence that the SPT6 protein is located in the nucleus.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Fluorescent Antibody Technique
  • Fungal Proteins / analysis
  • Fungal Proteins / genetics*
  • Genes, Fungal*
  • Genotype
  • Histone Chaperones
  • Molecular Sequence Data
  • Mutation
  • Nuclear Proteins / genetics*
  • Restriction Mapping
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*
  • Suppression, Genetic*
  • Transcription, Genetic*
  • Transcriptional Elongation Factors

Substances

  • Fungal Proteins
  • Histone Chaperones
  • Nuclear Proteins
  • SPT6 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcriptional Elongation Factors

Associated data

  • GENBANK/M34391