Label-free and sensitive thrombin sensing on a molecularly grafted aptamer on graphene

Yanping Wang; Yinghong Xiao; Xiaoling Ma; Na Li; Xiaodi Yang

doi:10.1039/c1cc15429j

Label-free and sensitive thrombin sensing on a molecularly grafted aptamer on graphene

Chem Commun (Camb). 2012 Jan 18;48(5):738-40. doi: 10.1039/c1cc15429j. Epub 2011 Nov 25.

Authors

Yanping Wang¹, Yinghong Xiao, Xiaoling Ma, Na Li, Xiaodi Yang

Affiliation

¹ Jiangsu Key Laboratory of New Power Batteries, College of Chemistry and Material Science, Nanjing Normal University, Nanjing 210097, China.

PMID: 22117200
DOI: 10.1039/c1cc15429j

Abstract

A unique nanocomposite was constructed by grafting an aptamer on graphene and used as a label-free biosensor for thrombin sensing, showing good selectivity, a low detection limit of 0.45 fM and high sensitivity, with a linear response up to 100 fM.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aptamers, Nucleotide / chemistry*
Aptamers, Nucleotide / metabolism
Biosensing Techniques / methods*
Chitosan / chemistry
Conductometry
Electrochemistry / methods*
Equipment Design
Glass / chemistry
Graphite / chemistry
Graphite / metabolism
Humans
Limit of Detection
Nanocomposites / chemistry*
Spectrum Analysis, Raman
Thrombin / analysis*

Substances

Aptamers, Nucleotide
Graphite
Chitosan
Thrombin