Restriction modification (RM) tests associated to additional molecular markers for screening prevalent MRSA clones in Brazil

C O Beltrame; A M N Botelho; M C Silva-Carvalho; R R Souza; R R Bonelli; M S Ramundo; M A Guimarães; L R Coelho; A M S Figueiredo

doi:10.1007/s10096-011-1534-1

Restriction modification (RM) tests associated to additional molecular markers for screening prevalent MRSA clones in Brazil

Eur J Clin Microbiol Infect Dis. 2012 Aug;31(8):2011-6. doi: 10.1007/s10096-011-1534-1. Epub 2012 Jan 13.

Authors

C O Beltrame¹, A M N Botelho, M C Silva-Carvalho, R R Souza, R R Bonelli, M S Ramundo, M A Guimarães, L R Coelho, A M S Figueiredo

Affiliation

¹ Instituto de Microbiologia Paulo de Góes, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho 373, Cidade Universitária, Rio de Janeiro, RJ, 21941-902, Brazil.

PMID: 22240854
DOI: 10.1007/s10096-011-1534-1

Abstract

In this study, we associated the restriction modification (RM) tests to the polymerase chain reaction (PCR) detection of molecular markers (SCCmec III, seh, agr II-SCCmec IV, and lukSF) for revealing the main methicillin-resistant Staphylococcus aureus (MRSA) clones circulating in Brazil. This simple and rapid approach allowed a precise classification of the MRSA analyzed when compared with pulsed-field gel electrophoresis (PFGE) data.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Brazil
Cluster Analysis
DNA Restriction-Modification Enzymes*
Electrophoresis, Gel, Pulsed-Field
Humans
Methicillin-Resistant Staphylococcus aureus / classification*
Methicillin-Resistant Staphylococcus aureus / genetics
Methicillin-Resistant Staphylococcus aureus / isolation & purification*
Molecular Typing / methods*
Polymerase Chain Reaction / methods*
Staphylococcal Infections / microbiology*

Substances

DNA Restriction-Modification Enzymes