Evaluation of new biomarker genes for differentiating Haemophilus influenzae from Haemophilus haemolyticus

M Jordan Theodore; Raydel D Anderson; Xin Wang; Lee S Katz; Jeni T Vuong; Melissa E Bell; Billie A Juni; Sara A Lowther; Ruth Lynfield; Jessica R MacNeil; Leonard W Mayer

doi:10.1128/JCM.06702-11

Evaluation of new biomarker genes for differentiating Haemophilus influenzae from Haemophilus haemolyticus

J Clin Microbiol. 2012 Apr;50(4):1422-4. doi: 10.1128/JCM.06702-11. Epub 2012 Feb 1.

Authors

M Jordan Theodore¹, Raydel D Anderson, Xin Wang, Lee S Katz, Jeni T Vuong, Melissa E Bell, Billie A Juni, Sara A Lowther, Ruth Lynfield, Jessica R MacNeil, Leonard W Mayer

Affiliation

¹ Meningitis and Vaccine Preventable Diseases Branch, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia, USA. ale7@cdc.gov

Abstract

PCR detecting the protein D (hpd) and fuculose kinase (fucK) genes showed high sensitivity and specificity for identifying Haemophilus influenzae and differentiating it from H. haemolyticus. Phylogenetic analysis using the 16S rRNA gene demonstrated two distinct groups for H. influenzae and H. haemolyticus.

MeSH terms

Bacterial Proteins / genetics
Carrier Proteins / genetics
Genetic Markers*
Haemophilus influenzae / genetics*
Immunoglobulin D / genetics
Lipoproteins / genetics
Molecular Typing
Phosphotransferases (Alcohol Group Acceptor) / genetics
Phylogeny
Polymerase Chain Reaction
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics

Substances

Bacterial Proteins
Carrier Proteins
Genetic Markers
Immunoglobulin D
Lipoproteins
RNA, Bacterial
RNA, Ribosomal, 16S
Phosphotransferases (Alcohol Group Acceptor)
glpQ protein, Haemophilus influenzae