Development and characterization of 20 new polymorphic microsatellite markers from Mangifera indica (Anacardiaceae)

Am J Bot. 2012 Mar;99(3):e117-9. doi: 10.3732/ajb.1100443. Epub 2012 Feb 27.

Abstract

Premise of the study: Twenty microsatellite loci for mango (Mangifera indica), an important commercial fruit tree in East Asia, were developed to evaluate the genetic diversity and identification of cultivars.

Methods and results: The 20 new microsatellite markers were isolated from mango using a magnetic bead enrichment method, and polymorphisms were identified in 22 mango cultivars. The number of alleles ranged from one to nine, with expected heterozygosity ranging from 0 to 0.826. The polymorphism information content ranged from 0 to 0.756 with a mean of 0.525.

Conclusions: These new microsatellite loci should be useful and convenient for further studies of the genetic diversity and identification of cultivars in mango.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Plant / genetics
  • Mangifera / genetics*
  • Microsatellite Repeats / genetics*
  • Polymerase Chain Reaction
  • Polymorphism, Genetic*

Substances

  • DNA, Plant