In this study, two-photon microscopy (TPM) was applied to obtain high-resolution, optically sectioned images with cellular morphology information from normal and cancerous nasopharyngeal tissue. Notable difference of nuclear-cytoplasmic ratio was found between these two groups. In particular, fractal dimension (FD) analysis based on TPM images showed good differentiation between normal and cancerous nasopharyngeal tissues. Our results indicate that TPM coupled with FD analysis methods has the potential to extract diagnostic features for label-free histological diagnosis.
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