Detection of cancer cells using a peptide nanotube-folic acid modified graphene electrode

Analyst. 2013 Feb 21;138(4):1026-31. doi: 10.1039/c2an36121c.

Abstract

This article describes the preparation of a graphene electrode modified with a new conjugate of peptide nanotubes and folic acid for the selective detection of human cervical cancer cells over-expressing folate receptors. The functionalization of peptide nanotubes with folic acid was confirmed by fluorescence microscopy and atomic force microscopy. The peptide nanotube-folic acid modified graphene electrode was characterized by scanning electron microscopy and cyclic voltammetry. The modification of the graphene electrode with peptide nanotube-folic acid led to an increase in the current signal. The human cervical cancer cells were bound to the modified electrode through the folic acid-folate receptor interaction. Cyclic voltammograms in the presence of [Fe(CN)(6)](3-/4-) as a redox species demonstrated that the binding of the folate receptor from human cervical cancer cells to the peptide nanotube-folic acid modified electrode lowered the electron transfer resulting in a decrease in the measured current. A detection limit of 250 human cervical cancer cells per mL was obtained. Control experiments confirmed that the peptide nanotube-folic acid electrode specifically recognized folate receptors. The modified electrode described here opens up new possibilities for future applications in early stage diagnoses of diseases where cells over-express folate receptors, such as in cancer or leishmaniasis disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrodes
  • Female
  • Folic Acid / chemistry*
  • Graphite / chemistry*
  • HeLa Cells
  • Humans
  • Nanotubes, Peptide / chemistry*
  • Uterine Cervical Neoplasms / chemistry*
  • Uterine Cervical Neoplasms / diagnosis

Substances

  • Nanotubes, Peptide
  • Graphite
  • Folic Acid