PPARγ regulates the mitochondrial dysfunction in human neural stem cells with tumor necrosis factor alpha

Neuroscience. 2013 Jan 15:229:118-29. doi: 10.1016/j.neuroscience.2012.11.003. Epub 2012 Nov 12.

Abstract

Peroxisome proliferator-activated receptor gamma (PPARγ) belongs to a family of ligand-activated transcription factors, and its ligands are known to control many physiological and pathological conditions. The hypothesis of our study was that the PPARγ agonist (rosiglitazone) could mediate tumor necrosis factor alpha (TNFα) related to the regulation of human neural stem cells (hNSCs), by which TNFα possibly fulfills important roles in neuronal impairment. The results show that PPARγ mediates the cell viability of hNSCs via the downregulation of the activity of caspase 3, indicating that this rescue effect of PPARγ could improve the reduced levels of two mitochondrial regulators, adenosine monophosphate-activated protein kinase (AMPK) and Sirtuin 1 (SIRT1) in the hNSCs with TNFα. The stimulation of mitochondrial function by PPARγ was associated with activation of the PPAR coactivator1 alpha (PGC1α) pathway by up-regulation of oxidative defense and mitochondrial systems. The above protective effects appeared to be exerted by a direct activation of the rosiglitazone, because it protected hNSCs from TNFα-evoked oxidative stress and mitochondrial deficiency. Here we show that the rosiglitazone protects hNSCs against Aβ-induced apoptosis and promotes cell survival. These findings extend our understanding of the central role of PPARγ in TNFα-related neuronal impairment, which probably increases risks of neurodegenerative diseases. The anti-inflammatory effects of PPARγ in the hNSCs with TNFα, and the involved mechanisms were also characterized.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Adenylate Kinase / metabolism
  • Caspase 3 / metabolism
  • Cell Survival / drug effects
  • Cell Survival / physiology
  • Chromans / pharmacology
  • Humans
  • Mitochondria / drug effects
  • Mitochondria / metabolism*
  • Neural Stem Cells / drug effects
  • Neural Stem Cells / metabolism*
  • Oxidative Stress / drug effects
  • Oxidative Stress / physiology
  • PPAR gamma / agonists
  • PPAR gamma / metabolism*
  • Pioglitazone
  • Reactive Oxygen Species / metabolism
  • Rosiglitazone
  • Signal Transduction / drug effects
  • Signal Transduction / physiology
  • Sirtuin 1 / metabolism
  • Thiazolidinediones / pharmacology
  • Troglitazone
  • Tumor Necrosis Factor-alpha / pharmacology*
  • Up-Regulation / drug effects
  • Up-Regulation / physiology

Substances

  • Chromans
  • PPAR gamma
  • Reactive Oxygen Species
  • Thiazolidinediones
  • Tumor Necrosis Factor-alpha
  • Rosiglitazone
  • Adenosine Triphosphate
  • Adenylate Kinase
  • Caspase 3
  • SIRT1 protein, human
  • Sirtuin 1
  • Troglitazone
  • Pioglitazone