Abstract
Transcriptional repression is a common approach to control gene expression in synthetic biology applications. Here, an engineered DNA binding protein based upon a transcription activator-like effector (TALE) scaffold was shown to outperform LacI in blocking transcription from a promoter and to repress expression of a downstream gene in an operon.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Bacteria / metabolism*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Lac Repressors / genetics
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Lac Repressors / metabolism
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Promoter Regions, Genetic
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Transcription, Genetic
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Xanthomonas / metabolism
Substances
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Bacterial Proteins
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DNA-Binding Proteins
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Lac Repressors