Synthesis of a photocaged tamoxifen for light-dependent activation of Cre-ER recombinase-driven gene modification

Chem Commun (Camb). 2013 May 28;49(43):4971-3. doi: 10.1039/c3cc42179a.

Abstract

We report the design of a water-soluble, quaternized tamoxifen photoprobe and demonstrate its application in light-controlled induction of green fluorescent protein expression via a Cre-ER recombinase system.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Estrogen Antagonists / chemistry*
  • Estrogen Antagonists / pharmacology
  • Estrogen Antagonists / radiation effects
  • Green Fluorescent Proteins / genetics
  • Integrases / genetics*
  • Mice
  • Nitrobenzenes / chemistry*
  • Nitrobenzenes / radiation effects
  • Receptors, Estrogen / genetics*
  • Recombination, Genetic
  • Tamoxifen / chemistry*
  • Tamoxifen / pharmacology
  • Tamoxifen / radiation effects
  • Ultraviolet Rays

Substances

  • Estrogen Antagonists
  • Nitrobenzenes
  • Receptors, Estrogen
  • Tamoxifen
  • Green Fluorescent Proteins
  • nitrobenzene
  • Cre recombinase
  • Integrases