Mitochondrial OXPHOS function is unaffected by chronic azithromycin treatment

Abstract

Background: Certain antibiotics may cause unwanted side effects due to the similarity of the mitochondrial translation system to the prokaryotic one. Children with cystic fibrosis (CF) are vulnerable to recurrent respiratory tract infections and azithromycin, a translation targeted antibiotic, is often used chronically to treat CF patients. No major clinical side effects were found with chronic treatment. However, mitochondrial function was not previously assessed. We evaluated oxidative phosphorylation (OXPHOS) in lymphocytes from children with CF receiving chronic azithromycin treatment using an improved ATP production assay.

Method: Enzymatic activities of respiratory chain complexes II-IV and ATP production were measured in lymphocytes.

Results: Relative to controls and to CF patients without azithromycin treatment, no significant difference in mitochondrial respiratory chain complexes II-IV was detected, and ATP production with pyruvate, glutamate and succinate, did not disclose any differences between the groups.

Conclusion: We suggest that chronic treatment with azithromycin does not significantly affect OXPHOS function.

Keywords: Azithromycin; Chronic treatment; Cystic fibrosis; Mitochondrial respiratory chain; OXPHOS.