Challenges of "sticky" co-immunoprecipitation: polyalanine tract protein-protein interactions

Methods Mol Biol. 2013:1017:121-33. doi: 10.1007/978-1-62703-438-8_9.

Abstract

Co-immunoprecipitation (Co-IP) (followed by immunoblotting) is a technique widely used to characterize specific protein-protein interactions. Investigating interactions of proteins containing "sticky" polyalanine (PolyA) tracts encounters difficulties using conventional Co-IP procedures. Here, we present strategies to specifically capture proteins containing these difficult PolyA tracts, enabling subsequent robust detection of interacting proteins by Co-IP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Homeodomain Proteins* / chemistry
  • Homeodomain Proteins* / isolation & purification
  • Homeodomain Proteins* / metabolism
  • Humans
  • Immunoprecipitation / methods*
  • Peptides* / chemistry
  • Peptides* / isolation & purification
  • Peptides* / metabolism
  • Transcription Factors* / chemistry
  • Transcription Factors* / isolation & purification
  • Transcription Factors* / metabolism

Substances

  • ARX protein, human
  • Homeodomain Proteins
  • Peptides
  • Transcription Factors
  • polyalanine