Investigation of bacterial nucleotide excision repair using single-molecule techniques

DNA Repair (Amst). 2014 Aug:20:41-48. doi: 10.1016/j.dnarep.2013.10.012. Epub 2014 Jan 25.

Abstract

Despite three decades of biochemical and structural analysis of the prokaryotic nucleotide excision repair (NER) system, many intriguing questions remain with regard to how the UvrA, UvrB, and UvrC proteins detect, verify and remove a wide range of DNA lesions. Single-molecule techniques have begun to allow more detailed understanding of the kinetics and action mechanism of this complex process. This article reviews how atomic force microscopy and fluorescence microscopy have captured new glimpses of how these proteins work together to mediate NER.

Keywords: Bacterial nucleotide excision repair; Single molecule; UvrA; UvrB; UvrC; UvrD.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Amino Acid Sequence
  • Bacillus / chemistry
  • Bacillus / enzymology
  • Bacillus / genetics
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Base Sequence
  • DNA / chemistry
  • DNA / metabolism
  • DNA Helicases / chemistry*
  • DNA Helicases / genetics
  • DNA Helicases / metabolism
  • DNA Repair Enzymes / chemistry*
  • DNA Repair Enzymes / genetics
  • DNA Repair Enzymes / metabolism
  • DNA Repair*
  • Microscopy, Fluorescence
  • Molecular Sequence Data

Substances

  • Bacterial Proteins
  • DNA
  • DNA Helicases
  • DNA Repair Enzymes