Fixed-bed biosorption of cadmium using immobilized Scenedesmus obliquus CNW-N cells on loofa (Luffa cylindrica) sponge

Bor-Yann Chen; Chun-Yen Chen; Wan-Qian Guo; Hao-Wei Chang; Wen-Ming Chen; Duu-Jong Lee; Chieh-Chen Huang; Nan-Qi Ren; Jo-Shu Chang

doi:10.1016/j.biortech.2014.02.006

Fixed-bed biosorption of cadmium using immobilized Scenedesmus obliquus CNW-N cells on loofa (Luffa cylindrica) sponge

Bioresour Technol. 2014 May:160:175-81. doi: 10.1016/j.biortech.2014.02.006. Epub 2014 Feb 11.

Authors

Bor-Yann Chen¹, Chun-Yen Chen², Wan-Qian Guo³, Hao-Wei Chang⁴, Wen-Ming Chen⁵, Duu-Jong Lee⁶, Chieh-Chen Huang⁷, Nan-Qi Ren³, Jo-Shu Chang⁸

Affiliations

¹ Department of Chemical and Materials Engineering, National I-Lan University, I-Lan, Taiwan.
² Center for Biosciences and Biotechnology, National Cheng Kung University, Tainan, Taiwan.
³ State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, PR China.
⁴ Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
⁵ Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd., Nan-Tzu, Kaohsiung City 811, Taiwan.
⁶ Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan.
⁷ Agricultural Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan; Department of Life Science, National Chung Hsing University, Taichung 402, Taiwan.
⁸ Center for Biosciences and Biotechnology, National Cheng Kung University, Tainan, Taiwan; Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan; Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan, Taiwan. Electronic address: changjs@mail.ncku.edu.tw.

PMID: 24581862
DOI: 10.1016/j.biortech.2014.02.006

Abstract

A continuous fixed-bed biosorption process was established for cadmium (Cd) removal by Scenedesmus obliquus CNW-N (isolated from southern Taiwan) cells immobilized onto loofa sponge. This immobilized-cell biosorption process allows better recovery and reusability of the microalgal biomass. The growth of microalgae on the matrix support with appropriate nutrient supplementation could enhance the overall metal removal activity. Major operating parameters (e.g., feeding flow rate, cycle number of medium replacement, and particle diameter of the sponge) were studied for treatability evaluation. The most promising cell growth on the sponge support was obtained at a flow rate of 0.284 bed volume (BV)/min, sponge particle diameter of 1 cm, and with one cycle of medium replacement. The performance of fixed-bed biosorption (adsorption capacity of 38.4 mg, breakthrough time at 15.5 h) was achieved at a flow rate of 5 ml/min with an influent concentration of 7.5 mg Cd/l.

Keywords: Biosorption; Cadmium; Fixed-bed process; Microalgal biosorbent; Scenedesmus obliquus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adsorption
Autotrophic Processes / drug effects
Biodegradation, Environmental / drug effects
Biomass
Cadmium / isolation & purification*
Carbon / pharmacology
Carbon Dioxide / pharmacology
Cells, Immobilized / cytology
Cells, Immobilized / drug effects
Cells, Immobilized / metabolism
Luffa / chemistry*
Microalgae / cytology
Microalgae / drug effects
Microalgae / growth & development
Rheology / drug effects
Scenedesmus / cytology*
Scenedesmus / drug effects
Scenedesmus / growth & development
Scenedesmus / metabolism*

Substances

Cadmium
Carbon Dioxide
Carbon