A new ganglioside of the lactotetraose series, GalNAc-3'-isoLM1, detected in human meconium

J Biol Chem. 1989 Jul 25;264(21):12122-5.

Abstract

A monoclonal antibody produced by immunization with cells of the human glioma cell line D-54 MG reacted with ganglioside GM2. The binding epitope of the antibody was found to be GalNAc beta 1-4(NeuAc alpha 2-3)Gal. Immunological detection of glycolipid antigens on thin-layer plates with this monoclonal antibody, DMAb-1, revealed the presence of a new ganglioside. This ganglioside, co-migrating with NeuAc alpha 2-6Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1Cer(6'-LM1) and GalNAc beta 1-4(NeuAc alpha 2-3)Gal beta 1-3GalNAc beta 1-4Gla beta 1-4Glc beta 1-1Cer (GalNAc-isoGM1) at chromatographic separation was isolated from human meconium. Its structure was determined by permethylation and fast atom bombardment-mass spectometry analyses. The new ganglioside was found to be a combination of the lacto and ganglio series gangliosides, and the structure found to be GalNAc beta 1-4(NeuAc alpha 2-3)Gal beta 1-3GlcNAc alpha 1-3Gal beta 1-4Glc beta 1-1Cer(GalNAc-3'-isoLM1).

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Monoclonal
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Epitopes / analysis
  • G(M2) Ganglioside / isolation & purification
  • Gangliosides / immunology
  • Gangliosides / isolation & purification*
  • Glycolipids / immunology
  • Humans
  • Infant, Newborn
  • Mass Spectrometry
  • Meconium / analysis*
  • Molecular Sequence Data

Substances

  • Antibodies, Monoclonal
  • Epitopes
  • Gangliosides
  • Glycolipids
  • GalNAc-3'-isoL(M1) ganglioside
  • G(M2) Ganglioside