Downregulation of PPP2R5E expression by miR-23a suppresses apoptosis to facilitate the growth of gastric cancer cells

FEBS Lett. 2014 Aug 25;588(17):3160-9. doi: 10.1016/j.febslet.2014.05.068. Epub 2014 Jul 2.

Abstract

PPP2R5E belongs to the phosphatase 2A regulatory subunit B family and acts as a tumor suppressor in human cancer. However, the role of PPP2R5E in the tumorigenesis of gastric cancer is unclear. Here, we declare that PPP2R5E is downregulated by miR-23a and induces cell growth inhibition and apoptosis in gastric cancer cells. Furthermore, ASO-miR-23a suppresses tumor growth derived from MGC803 cells in vivo. PPP2R5E is identified as a new target of miR-23a. Moreover, overexpression of PPP2R5E reversed the negative effects of miR-23a. We highlight the significance of miR-23a and PPP2R5E in the proliferation and apoptosis of gastric cancer cells.

Keywords: Apoptosis; Gastric cancer; PPP2R5E; Phosphatase 2A; Tumorigenesis; miR-23a.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / genetics*
  • Base Sequence
  • Carcinogenesis / genetics
  • Cell Line, Tumor
  • Cell Proliferation
  • Down-Regulation*
  • Humans
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism*
  • Protein Phosphatase 2 / genetics*
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / pathology*

Substances

  • MIRN23a microRNA, human
  • MicroRNAs
  • PPP2R5E protein, human
  • Protein Phosphatase 2