Freedom of expression: cell-type-specific gene profiling

Wiley Interdiscip Rev Dev Biol. 2014 Nov-Dec;3(6):429-43. doi: 10.1002/wdev.149. Epub 2014 Aug 29.

Abstract

Cell fate and behavior are results of differential gene regulation, making techniques to profile gene expression in specific cell types highly desirable. Many methods now enable investigation at the DNA, RNA and protein level. This review introduces the most recent and popular techniques, and discusses key issues influencing the choice between these such as ease, cost and applicability of information gained. Interdisciplinary collaborations will no doubt contribute further advances, including not just in single cell type but single-cell expression profiling.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Cell Nucleus / metabolism
  • Cell Separation
  • Gene Expression Profiling*
  • Gene Expression Regulation, Developmental
  • Humans
  • Organ Specificity / genetics*