β-Carboline alkaloids from Galianthe ramosa inhibit malate synthase from Paracoccidioides spp

Planta Med. 2014 Dec;80(18):1746-52. doi: 10.1055/s-0034-1383305. Epub 2014 Nov 20.

Abstract

As part of our continuing chemical and biological analyses of Rubiaceae species from Cerrado, we isolated novel alkaloids 1 and 2, along with known compounds epicatechin, ursolic acid, and oleanolic acid, from Galianthe ramosa. Alkaloid 2 inhibited malate synthase from the pathogenic fungus Paracoccidioides spp. This enzyme is considered an important molecular target because it is not found in humans. Molecular docking simulations were used to describe the interactions between the alkaloids and malate synthase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaloids / chemistry
  • Alkaloids / pharmacology
  • Antifungal Agents / chemistry
  • Antifungal Agents / pharmacology*
  • Carbolines / chemistry
  • Carbolines / pharmacology*
  • Enzyme Inhibitors / chemistry
  • Enzyme Inhibitors / pharmacology*
  • Fungal Proteins / metabolism
  • Inhibitory Concentration 50
  • Malate Synthase / antagonists & inhibitors*
  • Malate Synthase / chemistry
  • Malate Synthase / metabolism
  • Microbial Sensitivity Tests
  • Molecular Docking Simulation
  • Molecular Structure
  • Paracoccidioides / drug effects
  • Paracoccidioides / enzymology*
  • Paracoccidioides / pathogenicity
  • Plant Components, Aerial / chemistry
  • Rubiaceae / chemistry

Substances

  • Alkaloids
  • Antifungal Agents
  • Carbolines
  • Enzyme Inhibitors
  • Fungal Proteins
  • Malate Synthase