A plaque hybridization assay for rotaviruses

J Virol Methods. 1989 Oct;26(1):27-33. doi: 10.1016/0166-0934(89)90071-2.

Abstract

A plaque hybridization assay was adapted to rotavirus. In this test cDNA or oligonucleotide probes were used to discriminate between plaques originating from virus carrying genes of bovine and simian origin. Only mRNAs present in infected cells were detected as demonstrated by using oligonucleotides corresponding to both strands. This assay can be used to screen reassortants or mutants of rotaviruses.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cells, Cultured
  • Chlorocebus aethiops
  • DNA Probes
  • Genes, Viral
  • Kidney
  • Molecular Sequence Data
  • Nucleic Acid Hybridization*
  • Oligonucleotide Probes
  • RNA, Messenger / analysis*
  • RNA, Viral / analysis*
  • Rotavirus / classification
  • Rotavirus / genetics
  • Rotavirus / isolation & purification*
  • Sequence Homology, Nucleic Acid
  • Viral Plaque Assay / methods*

Substances

  • DNA Probes
  • Oligonucleotide Probes
  • RNA, Messenger
  • RNA, Viral