Using gene therapy to manipulate the immune system in the fight against B-cell leukemias

Expert Opin Biol Ther. 2015 Mar;15(3):403-16. doi: 10.1517/14712598.2015.1014792. Epub 2015 Feb 9.

Abstract

Introduction: Over 20 years ago, chimeric antigen receptors (CARs) were created to endow T cells with new antigen-specificity and create a therapy that could eradicate cancer and provide life-long protection against recurrence. Steady progress has led to significant improvements with CAR design and CAR T-cell production, allowing evaluation of CAR T cells in patients. The initial trials have targeted CD19, which is expressed on normal and malignant B cells.

Areas covered: We review data from trials for patients with chronic lymphocytic leukemia (CLL) and B-cell acute lymphoblastic leukemia (B-ALL). In addition, we discuss the on-target toxicities, B-cell aplasia and cytokine release syndrome (CRS), which is uniquely associated with T-cell immunotherapies.

Expert opinion: We compare the results when targeting the same antigen in CLL or B-ALL and speculate on reasons for outcome differences and future directions to enhance outcomes. Furthermore, the dramatic results targeting B-ALL require further analysis in Phase II trials, and we discuss important components of these future trials. We also suggest a management scheme for CRS. The next several years will be critical and may lead to the first clinical indication of a gene-engineered cell therapy for cancer.

Keywords: B-cell acute lymphoblastic leukemia; adoptive T-cell therapy; chimeric antigen receptors; chronic lymphocytic leukemia; gene therapy; immunotherapy.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Antigens, CD19 / genetics
  • Antigens, CD19 / immunology
  • B-Lymphocytes / immunology*
  • Genetic Therapy / methods*
  • Humans
  • Immunotherapy, Adoptive / methods
  • Leukemia, B-Cell / genetics
  • Leukemia, B-Cell / immunology*
  • Leukemia, B-Cell / therapy*
  • Leukemia, Lymphocytic, Chronic, B-Cell / genetics
  • Leukemia, Lymphocytic, Chronic, B-Cell / immunology
  • Leukemia, Lymphocytic, Chronic, B-Cell / therapy
  • Receptors, Antigen / genetics
  • Receptors, Antigen / immunology
  • T-Lymphocytes / immunology

Substances

  • Antigens, CD19
  • Receptors, Antigen