[Development of noninvasive bladder cancer diagnosis on basis of telomerase and it's subunits hTERT and hTR detection]

Biomed Khim. 2015 Jan-Feb;61(1):150-60. doi: 10.18097/pbmc20156101150.
[Article in Russian]

Abstract

Telomerase activity (TA) and expression of genes coding it's subunits (hTERT and hTR) have been examined in tumor tissue and urine sediment samples taken from patients with bladder cancer (BC) using the modified TRAP assay (in the case of telomerase detection) and RT-PCR (in the case of hTERT and hTR expression). Results obtained in this study demonstrate possibility of noninvasive diagnosis of BC with sensitivity of 96% and specificity of 100% in the case of telomerase detection and with sensitivity of 80% and specificity of 100% in the case of hTERT detection in urine sediment samples.

Dlia razrabotki metodiki neinvazivnoĭ diagnostiki raka mochevogo puzyria byl proveden analiz aktivnosti telomerazy v obraztsakh tkani opukholi mochevogo puzyria i kletochnykh osadkakh mochi patsientov neizotopnym metodom TRAP (telomerase repeat amplification protocol). Takzhe byli izmereny urovni otnositel'noĭ ékspressii ee kataliticheskiĭ sub"edinitsy hTERT i RNK-sub"edinitsy (hTR) metodom OT-PTsR. Aktivnost' telomerazy i ékspressiia genov, kodiruiushchikh ee sub"edinitsy, vyiavlialas' kak v tkani opukholi, tak i v kletochnom osadke mochi kazhdogo patsienta. Dannye, poluchennye nami, pokazyvaiut vozmozhnost' provedeniia neinvazivnoĭ diagnostiki raka mochevogo puzyria s ispol'zovaniem kombinatsii ukazannykh metodov s chuvstvitel'nost'iu i spetsifichnost'iu v 96 v 100% dlia analiza AT i 80 i 100% dlia analiza ékspressii gena hTERT.

Publication types

  • English Abstract

MeSH terms

  • Biomarkers, Tumor / genetics
  • Biomarkers, Tumor / metabolism*
  • Biomarkers, Tumor / urine
  • Case-Control Studies
  • Humans
  • Protein Subunits / genetics
  • Protein Subunits / metabolism
  • Protein Subunits / urine
  • Sensitivity and Specificity
  • Telomerase / genetics
  • Telomerase / metabolism*
  • Telomerase / urine
  • Urinary Bladder Neoplasms / diagnosis*
  • Urinary Bladder Neoplasms / metabolism

Substances

  • Biomarkers, Tumor
  • Protein Subunits
  • TERT protein, human
  • Telomerase