Overexpression of miR-145-5p inhibits proliferation of prostate cancer cells and reduces SOX2 expression

Cancer Invest. 2015 Jul;33(6):251-8. doi: 10.3109/07357907.2015.1025407. Epub 2015 May 7.

Abstract

We aimed to perform functional analysis of miR-145-5p in prostate cancer (PCa) cells and to identify targets of miR-145-5p for understanding its role in PCa pathogenesis. PC3, DU145, LNCaP PCa, and PNT1a nontumorigenic prostate cell lines were utilized for functional analysis of miR-145-5p. Its overexpression caused inhibition of proliferation through apoptosis and reduced migration in PCa cells. SOX2 expression was significantly decreased in both mRNA and protein level in miR-145-5p-overexpressed PCa cells. We proposed that miR-145-5p, being an important regulator of SOX2, carries a crucial role in PCa tumorigenesis.

Keywords: Cancer stem cell; Prostate cancer; SOX2; Stem cell marker; hsa-miR-145–5p.

MeSH terms

  • Apoptosis / physiology
  • Blotting, Western
  • Cell Line, Tumor
  • Cell Proliferation* / physiology
  • Gene Expression Regulation, Neoplastic / physiology*
  • Humans
  • Male
  • MicroRNAs / biosynthesis*
  • Oligonucleotide Array Sequence Analysis
  • Prostatic Neoplasms / genetics
  • Prostatic Neoplasms / metabolism
  • Prostatic Neoplasms / pathology*
  • Real-Time Polymerase Chain Reaction
  • SOXB1 Transcription Factors / biosynthesis*

Substances

  • MIRN145 microRNA, human
  • MicroRNAs
  • SOX2 protein, human
  • SOXB1 Transcription Factors