Detection of enteropathogens associated with travelers' diarrhea using a multiplex Luminex-based assay performed on stool samples smeared on Whatman FTA Elute cards

Tahaniyat Lalani; Michele D Tisdale; Jason D Maguire; Chansuda Wongsrichanalai; Mark S Riddle; David R Tribble

doi:10.1016/j.diagmicrobio.2015.05.011

Detection of enteropathogens associated with travelers' diarrhea using a multiplex Luminex-based assay performed on stool samples smeared on Whatman FTA Elute cards

Diagn Microbiol Infect Dis. 2015 Sep;83(1):18-20. doi: 10.1016/j.diagmicrobio.2015.05.011. Epub 2015 May 27.

Authors

Tahaniyat Lalani¹, Michele D Tisdale², Jason D Maguire³, Chansuda Wongsrichanalai⁴, Mark S Riddle⁵, David R Tribble⁶

Affiliations

¹ Infectious Disease Clinic, Naval Medical Center, Portsmouth, VA, USA; Infectious Disease Clinical Research Program, Uniformed Services University, Bethesda, MD, USA; Henry M Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA. Electronic address: tlalani@idcrp.org.
² Infectious Disease Clinic, Naval Medical Center, Portsmouth, VA, USA; Infectious Disease Clinical Research Program, Uniformed Services University, Bethesda, MD, USA; Henry M Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
³ Infectious Disease Clinic, Naval Medical Center, Portsmouth, VA, USA.
⁴ Independent Consultant, San Angelo, TX, USA.
⁵ Enteric Diseases Department, Naval Medical Research Center, Silver Spring, MD, USA.
⁶ Infectious Disease Clinical Research Program, Uniformed Services University, Bethesda, MD, USA.

Abstract

We evaluated the limits of detection (LoD) for an 11-plex PCR-Luminex assay performed on Whatman(™) FTA Elute cards smeared with stool containing pathogens associated with travelers' diarrhea. LoDs ranged from 10(2) to 10(5)CFU, PFU, or cysts/g for most pathogens except Cryptosporidium. Campylobacter and norovirus LoDs increased with prolonged storage of cards.

Keywords: PCR; Stool card; Travelers' diarrhea.

Publication types

Evaluation Study
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Campylobacter / isolation & purification
Cryptosporidium / isolation & purification
Diarrhea / diagnosis*
Diarrhea / microbiology
Diarrhea / parasitology
Diarrhea / virology
Feces / microbiology*
Feces / parasitology
Feces / virology*
Microbiological Techniques / methods*
Molecular Diagnostic Techniques / methods*
Norovirus / isolation & purification
Pilot Projects
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Travel*

Abstract

Publication types

MeSH terms

Grants and funding