Mass spectrometrists should search only for peptides they care about

Nat Methods. 2015 Jul;12(7):605-8. doi: 10.1038/nmeth.3450.

Abstract

Analysis pipelines that assign peptides to shotgun proteomics mass spectra often discard identified spectra deemed irrelevant to the scientific hypothesis being tested. To improve statistical power, I propose that researchers remove irrelevant peptides from the database prior to searching rather than assigning these peptides to spectra and then discarding the matches.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Humans
  • Mass Spectrometry*
  • Peptides / analysis
  • Plasmodium / chemistry
  • Proteomics / methods*

Substances

  • Peptides